This course provides a comprehensive overview of DOT drug testing requirements under 49 CFR Parts 40 and 382, designed for commercial drivers and those responsible for DOT compliance. Participants will learn when and why drug tests are required, the substances tested, proper testing procedures, Clearinghouse responsibilities, and the consequences of violations for both drivers and employers. The course also covers driver and employer responsibilities, reasonable suspicion awareness, and the return-to-duty process, helping organizations maintain a compliant, safe, and drug-free workplace.